ION-SPRAY MASS-SPECTROMETRY OF MARINE TOXINS .2. ANALYSIS OF DIARRHETIC SHELLFISH TOXINS IN PLANKTON BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：70

作者：

PLEASANCE, S ^{[1
]}

QUILLIAM, MA ^{[1
]}

DEFREITAS, ASW ^{[1
]}

MARR, JC ^{[1
]}

CEMBELLA, AD ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,ATLANTIC REG LAB,1411 OXFORD ST,HALIFAX B3H 3Z1,NS,CANADA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 06期

关键词：

D O I：

10.1002/rcm.1290040608

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A combined liquid chromatography/mass spectrometry (LC/MS) method using ion‐spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin‐1, the principal toxins implicated in cases of diarrhetic shellfish poisoning. The method is used to confirm the presence of okadaic acid in a culture of the dinoflagellate species Prorocentrum concavum and in natural populations of dinoflagellates from eastern Canadian waters. Several analogues of the toxin have also been detected. Quantitative results are comparable to those obtained by high performance liquid chromatography with fluorescence detection. With slight modifications, the LC/MS method is also used to analyse the fluorescent anthrylmethyl ester of okadaic acid. Preliminary tandem mass spectrometric results, obtained using fast‐atom bombardment ionization, are presented for okadaic acid. Copyright © 1990 John Wiley & Sons Limited

引用

页码：206 / 213

页数：8

共 22 条

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