LIVE SHIGELLA-FLEXNERI 2A AND SHIGELLA-SONNEI I-VACCINE CANDIDATE STRAINS WITH 2 ATTENUATING MARKERS .1. CONSTRUCTION OF VACCINE CANDIDATE STRAINS WITH RETAINED INVASIVENESS BUT REDUCED INTRACELLULAR MULTIPLICATION

被引:23
作者
LINDE, K
DENTCHEV, V
BONDARENKO, V
MARINOVA, S
RANDHAGEN, B
BRATOYEVA, M
TSVETANOV, Y
ROMANOVA, Y
机构
[1] ACAD MED SOFIA, INFECT & PARASIT DIS RES INST, SOFIA, BULGARIA
[2] NF GAMALEYA EPIDEMIOL & MICROBIOL RES INST, MOSCOW, USSR
关键词
attenuation; live vaccine; Shigella flexneri 2a; Shigella sonnei I;
D O I
10.1016/0264-410X(90)90173-J
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Live Shigella flexneri 2a and Shigella sonnei Phase I vaccine candidate strains with two virulence-reducing markers were constructed through stepwise incorporation of weakly attenuating purine auxotrophy with subsequent rifampicin-resistance (RNA polymerase) mutation to yield optimal attenuation. These vaccine candidate strains showed an unaltered plasmid profile; did not cause keratoconjunctivitis in the Sereny test, while being excreted for a short but still marked period and providing partial protection from wild-strain infection; exhibited for guinea-pig conjunctival epithelia, HeLa cells and rat enterocytes a maintained invasiveness with reduced intracellular multiplication with little, if any, reversible cell damage; and produced, just as their ultrasonic lysates, no exudative reaction in the rabbit gut loop test. © 1990.
引用
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页码:25 / 29
页数:5
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