COMPARISONS OF SOLUBLE-PROTEINS AND ISOZYMES FOR 7 PYTHIUM SPECIES AND APPLICATIONS OF THE BIOCHEMICAL DATA TO PYTHIUM SYSTEMATICS

被引:26
作者
CHEN, WD [1 ]
HOY, JW [1 ]
SCHNEIDER, RW [1 ]
机构
[1] LOUISIANA STATE UNIV,LOUISIANA AGR EXPT STN,DEPT PLANT PATHOL & CROP PHYSIOL,CTR AGR,BATON ROUGE,LA 70803
来源
MYCOLOGICAL RESEARCH | 1991年 / 95卷
关键词
D O I
10.1016/S0953-7562(09)80067-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Seven homothallic Pythium species were compared using SDS-polyacrylamide gel electrophoresis of soluble proteins and starch gel isozyme analysis. Similarities in soluble protein banding patterns and isozyme phenotypes were detected for morphologically distinct species. However, morphologically similar species (P. aphanidermatum vs. P. deliense and P. arrhenomanes vs. P. graminicola) were not always distinguishable. Cluster and principle component analysis of data from both soluble proteins and isozymes generally grouped the isolates by species. Levels of intraspecific variation differed between species with each technique. Implications of the results from protein electrophoresis and isozyme analysis for Pythium systematics are discussed.
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页码:548 / 555
页数:8
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