A MOLECULAR-MODEL FOR MEMBRANE-FUSION BASED ON SOLUTION STUDIES OF AN AMPHIPHILIC PEPTIDE FROM HIV GP41

被引:44
作者
FUJII, G
HORVATH, S
WOODWARD, S
EISERLING, F
EISENBERG, D
机构
[1] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA
[2] UNIV CALIF LOS ANGELES, DEPT CHEM & BIOCHEM, LOS ANGELES, CA 90024 USA
[3] UNIV CALIF LOS ANGELES, DEPT MICROBIOL & MOLEC GENET, LOS ANGELES, CA 90024 USA
[4] VESTAR INC, SAN DIMAS, CA 91773 USA
[5] CALTECH, CTR BIOPOLYMER SYNTH, PASADENA, CA 91125 USA
关键词
HUMAN IMMUNODEFICIENCY VIRUS; LIPID BINDING; MEMBRANE FUSION; PROTEIN MEMBRANE INTERACTIONS;
D O I
10.1002/pro.5560011107
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mechanism of protein-mediated membrane fusion and lysis has been investigated by solution-state studies of the effects of peptides on liposomes. A peptide (SI) corresponding to a highly amphiphilic C-terminal segment from the envelope protein (gp41) of the human immunodeficiency virus (HIV) was synthesized and tested for its ability to cause lipid membranes to fuse together (fusion) or to break open (lysis). These effects were compared to those produced by the lytic and fusogenic peptide from bee venom, melittin. Other properties studied included the changes in visible absorbance and mean particle size, and the secondary structure of peptides as judged by CD spectroscopy. Taken together, the observations suggest that protein-mediated membrane fusion is dependent not only on hydrophobic and electrostatic forces but also on the spatial arrangement of the amino acid residues to form an amphiphilic structure that promotes the mixing of the lipids between membranes. A speculative molecular model is proposed for membrane fusion by alpha-helical peptides, and its relationship to the forces involved in protein-membrane interactions is discussed.
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页码:1454 / 1464
页数:11
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