MICROMACHINED ENZYME REACTOR FOR FIA SYSTEM

An enzyme reactor consisting of a 2.6-m-long silicon capillary with glucose oxidase immobilized on the inner surface was fabricated using micromachining techniques. A V-shaped groove of 100 mum width, formed by anisotropic etching, was anodically bonded to a glass plate to create the capillary. Glucose oxidase was covalently immobilized with 3-aminopropyltriethoxysilane and glutaraldehyde. The reactor was evaluated by connecting it to a Flow injection analysis system for glucose detection. Glucose concentrations were in the range of 10(-3) to 5 x 10(-2) M with a volume of 0.2 mul of glucose solution. (C) 1994 Academic Press, Inc.