RECOVERY OF PROTEINS AND AMINO-ACIDS FROM REVERSE MICELLES BY DEHYDRATION WITH MOLECULAR-SIEVES

被引：28

作者：

GUPTA, RB ^{[1
]}

HAN, CJ ^{[1
]}

JOHNSTON, KP ^{[1
]}

机构：

[1] UNIV TEXAS,DEPT CHEM ENGN,AUSTIN,TX 78712

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1994年 / 44卷 / 07期

关键词：

REVERSE MICELLES; PROTEIN PRECIPITATION; AMINO ACID PRECIPITATION;

D O I：

10.1002/bit.260440708

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new method is presented to precipitate proteins and amino acids from reverse micelles by dehydrating the micelles with molecular sieves. Nearly complete precipitation is demonstrated for alpha-chymotrypsin, cytochrome-c, and tryptophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isooctane/water reverse micelle solutions. The products precipitate as a solid powder, which is relatively free of surfactant. The method does not require any manipulation of pH, ionic strength, temperature, pressure, or solvent composition, and is applicable over a broad range of these properties. This general approach is compared with other techniques for the recovery of biomolecules from reverse micelles. (C) 1994 John Wiley & Sons, Inc.

引用

页码：830 / 836

页数：7

共 34 条

[1] EXTRACTION OF AMINO-ACIDS TO MICROEMULSION [J].