DIFFERENTIAL-EFFECTS OF AMYLOID PEPTIDES BETA-(1-40) AND BETA-(25-35) INJECTIONS INTO THE RAT NUCLEUS BASALIS

被引：165

作者：

GIOVANNELLI, L ^{[1
]}

CASAMENTI, F ^{[1
]}

SCALI, C ^{[1
]}

BARTOLINI, L ^{[1
]}

PEPEU, G ^{[1
]}

机构：

[1] UNIV FLORENCE, DEPT PRECLIN & CLIN PHARMACOL, I-50134 FLORENCE, ITALY

来源：

NEUROSCIENCE | 1995年 / 66卷 / 04期

关键词：

D O I：

10.1016/0306-4522(94)00610-H

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The nucleus basalis of male Charles River Wistar rats was injected with 10 mu g of the beta-amyloid peptides beta-(1-40) and beta-(25-35) and changes in the morphology of the lesioned area, the release of acetylcholine from the cortex, and in behavior were investigated. Injections of saline and a scrambled (25-35) peptide were used as controls. One week after lesioning, a Congo Red-positive deposit of aggregated material was found at the beta-peptides injection site, which lasted for about 21 days in the case of the beta-(25-35) peptide and at least two months for beta-(1-40). No deposit was detected after scrambled peptide injection. At one week post injection, an extensive glial reaction surrounded the injection site of all peptides and saline as well. Such a reaction was still present but rather attenuated after two months. A decrease in the number of cholinergic neurons was detected in the nucleus basalis after one week with all treatments except saline. After two months, a reduction in the number of choline acetyltransferase-immunopositive neurons was still detectable in the rats injected with beta-(1-40) but not in the beta-(25-35)- or scrambled-injected. The reduction in choline acetyltransferase immunoreactivity was closely paralleled by a decrease in basal acetylcholine release from the parietal dorter ipsilateral to the lesion. Disruption of object recognition was observed in the first weeks after beta-(25-35) peptide injection, whereas the beta-(1-40) peptide impaired the performance only two months after lesion. Rats with lesions induced by beta-peptides may be a useful animal model of amyloid deposition for investigation of the pathogenetic mechanisms leading to Alzheimer's disease.

引用

页码：781 / 792

页数：12

共 40 条

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