ANTIFUNGAL ANTIBIOTIC OF BASIDIOMYCETE OUDEMANSIELLA MUCIDA .I. ISOLATION AND CULTIVATION OF A PRODUCING STRAIN

A pure culture of the basidiomycete Oudemansiella mucida, isolated by the explantation technique, produces an intracellular antifungal antibiotic. The relatively simple basic growth requirements of the producing strain permit its cultivation on agar and liquid nutrient media. During submerse cultivation in medium containing glucose and cornsteep on a shaker apparatus or in a fermentor, the fungus produces 300-600 μg antibiotic/ml. The main growth characteristics of a mycelial culture of the producing strain and details of its transfer to submerse conditions are described. © 1969 Institute of Microbiology, Academy of Sciences of the Czech Republic.