5-FORMYLDEOXYURIDINE - A NEW TYPE OF DNA DAMAGE INDUCED BY IONIZING-RADIATION AND ITS MUTAGENICITY TO SALMONELLA STRAIN TA102

被引：116

作者：

KASAI, H ^{[1
]}

IIDA, A ^{[1
]}

YAMAIZUMI, Z ^{[1
]}

NISHIMURA, S ^{[1
]}

TANOOKA, H ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV RADIOBIOL,CHUO KU,TOKYO 104,JAPAN

来源：

MUTATION RESEARCH | 1990年 / 243卷 / 04期

关键词：

5-Formyldeoxyuridine; DNA damage; Ionizing radiation;

D O I：

10.1016/0165-7992(90)90139-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An aqueous solution of calf thymus DNA was irradiated by 60Co γ-rays and modified nucleosides produced in DNA were analyzed by high-pressure liquid chromatography coupled with a photodiode array UV detector. A new product with UV absorption maxima at 230 nm and 280 nm was observed. The structure of this compound was proposed to be 5-formyldeoxyuridine (f5dU) based on the mass spectrum of its trimethylsilyl derivative (M+, m/z 472) and the structure was confirmed by chemical synthesis. The yield of f5dU ( 2.4 104 dT/krad) in DNA was of roughly the same order as that of 8-hydroxydeoxyguanosine and 5-hydroxymethyldeoxyuridine. Free f5dU was mutagenic to Salmonella typhimurium strain TA102: therefore f5dU incorporated into DNA may induce mutations. © 1990.

引用

页码：249 / 253

页数：5

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