RHIZOBIUM-MELILOTI CHROMOSOMAL LOCI REQUIRED FOR SUPPRESSION OF EXOPOLYSACCHARIDE MUTATIONS BY LIPOPOLYSACCHARIDE

被引：14

作者：

WILLIAMS, MNV

HOLLINGSWORTH, RI

BRZOSKA, PM

SIGNER, ER

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

[2] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 11期

关键词：

D O I：

10.1128/jb.172.11.6596-6598.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutants of alfalfa symbiont Rhizobium meliloti SU47 that fail to make extracellular polysaccharide (exo mutants) induce the formation of nodules that are devoid of bacteria and consequently do not fix nitrogen. This Fix- phenotype can be suppressed by an R. meliloti Rm41 gene that affects lipopolysaccharide structure. Here we describe mutations preventing suppression that map at two new chromosomal loci, lpsY and lpsX, present in both strains. Two other lps mutations isolated previously from SU47 also prevented suppression.

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页码：6596 / 6598

页数：3

相关论文

共 19 条

[1] THE PROKARYOTIC TRANSPOSABLE ELEMENT TN5 [J].

BERG, DE ;

BERG, CM .

BIO-TECHNOLOGY, 1983, 1 (05) :417-435

[2] ALTERATION OF THE EFFECTIVE NODULATION PROPERTIES OF A FASTGROWING BROAD HOST RANGE RHIZOBIUM DUE TO CHANGES IN EXOPOLYSACCHARIDE SYNTHESIS [J].

CHEN, H ;

BATLEY, M ;

REDMOND, J ;

ROLFE, BG .

JOURNAL OF PLANT PHYSIOLOGY, 1985, 120 (04) :331-349

[3] LIPOPOLYSACCHARIDE MUTANTS OF RHIZOBIUM-MELILOTI ARE NOT DEFECTIVE IN SYMBIOSIS [J].

CLOVER, RH ;

KIEBER, J ;

SIGNER, ER .

JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :3961-3967

[4] GENETIC MANIPULATIONS IN RHIZOBIUM-MELILOTI UTILIZING 2 NEW TRANSPOSON TN5 DERIVATIVES [J].

DEVOS, GF ;

WALKER, GC ;

SIGNER, ER .

MOLECULAR & GENERAL GENETICS, 1986, 204 (03) :485-491

[5] SYMBIOTIC MUTANTS OF RHIZOBIUM MELILOTI THAT UNCOUPLE PLANT FROM BACTERIAL DIFFERENTIATION [J].

FINAN, TM ;

HIRSCH, AM ;

LEIGH, JA ;

JOHANSEN, E ;

KULDAU, GA ;

DEEGAN, S ;

WALKER, GC ;

SIGNER, ER .

CELL, 1985, 40 (04) :869-877

[6] 2ND SYMBIOTIC MEGAPLASMID IN RHIZOBIUM-MELILOTI CARRYING EXOPOLYSACCHARIDE AND THIAMINE SYNTHESIS GENES [J].

FINAN, TM ;

KUNKEL, B ;

DEVOS, GF ;

SIGNER, ER .

JOURNAL OF BACTERIOLOGY, 1986, 167 (01) :66-72

[7] A NOVEL EXOPOLYSACCHARIDE CAN FUNCTION IN PLACE OF THE CALCOFLUOR-BINDING EXOPOLYSACCHARIDE IN NODULATION OF ALFALFA BY RHIZOBIUM-MELILOTI [J].

GLAZEBROOK, J ;

WALKER, GC .

CELL, 1989, 56 (04) :661-672

[8]

KHANUJA SPS, J GENET, V68, P93

[9] EXOPOLYSACCHARIDE-DEFICIENT MUTANTS OF RHIZOBIUM-MELILOTI THAT FORM INEFFECTIVE NODULES [J].

LEIGH, JA ;

SIGNER, ER ;

WALKER, GC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (18) :6231-6235

[10] CLONING OF RHIZOBIUM-MELILOTI NODULATION GENES BY DIRECT COMPLEMENTATION OF NOD- MUTANTS [J].

LONG, SR ;

BUIKEMA, WJ ;

AUSUBEL, FM .

NATURE, 1982, 298 (5873) :485-488