HYDROPHOBIC ESTERS OF CELLULOSE - PROPERTIES AND APPLICATIONS IN BIOCHEMICAL TECHNOLOGY

被引：14

作者：

DIXON, J

ANDREWS, P

BUTLER, LG

机构：

[1] Department of Biochemistry, Purdue University, West Lafayette, Indiana

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 11期

关键词：

D O I：

10.1002/bit.260211115

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have investigated the utility for enzyme immobilization of several hydrophobic cellulose esters, as a function of solvent composition, extent of esterification, and enzyme. Phenoxyacetyl cellulose was also used for immobilization of rat liver microsomes, hydrophobic chromatography of proteins, and removal of Triton X‐100 from protein solutions. Phenoxyacetyl groups esterified to cellulose were much less subject to enzymatic hydrolysis than soluble phenoxyacetyl esters. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：2113 / 2123

页数：11

共 15 条

[1] Porath J., Caldwell K.D., Biotechnical Applications of Proteins and Enzymes, (1977)
[2] Butler L.G., Arch. Biochem. Biophys., 171, (1975)
[3] Andrews P.A., (1978)
[4] Brown M.S., Dana S.E., Dietschy J.M., Siperstein M.D., J. Bioi. Chem., 248, (1973)
[5] Sperow J., Butler L.G., Arch. Biochem. Biophys., 146, (1971)
[6] Bennett V., Hamming J.J., Butler L.G., Maize leaf inorganic pyrophosphatase: isozymes, specificity for substrates, inhibitors, and divalent metal ions, and pH optima., Biochim Biophys Acta, 293, (1973)
[7] Hestrin S., J. Biol. Chem., 180, (1947)
[8] Lipman F., Tuttle L.G., J. Bioi. Chem., 159, (1945)
[9] Reynolds J.A., Herbert S., Polet H., Steinhardt J., Biochemistry, 6, (1967)
[10] Hofstee B.H.J., Biochem. Biophys. Res. Commun., 63, (1975)

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