MOLECULAR-CLONING AND CHARACTERIZATION OF THE GENE ENCODING THE ADENINE METHYLTRANSFERASE M-CVIRI FROM CHLORELLA-VIRUS XZ-6E

被引：23

作者：

STEFAN, C ^{[1
]}

XIA, YN ^{[1
]}

VANETTEN, JL ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT PLANT PATHOL,LINCOLN,NE 68583

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 02期

关键词：

D O I：

10.1093/nar/19.2.307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypetide of 379 amino acids with a predicted molecular weight of 42,814. Comparison of the M.CviRI predicted amino acid sequence with another Chlorella virus and 14 bacterial adenine methyltransferases revealed extensive similarity to the other Chlorella virus enzyme.

引用

收藏

页码：307 / 311

页数：5

相关论文

共 44 条

[1] CHARACTERIZATION OF THE GENES-CODING FOR THE ECO RV RESTRICTION AND MODIFICATION SYSTEM OF ESCHERICHIA-COLI [J].

BOUGUELERET, L ;

SCHWARZSTEIN, M ;

TSUGITA, A ;

ZABEAU, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (08) :3659-3676

[2] THE ISOLATION AND CHARACTERIZATION OF THE ESCHERICHIA-COLI DNA ADENINE METHYLASE (DAM) GENE [J].

BROOKS, JE ;

BLUMENTHAL, RM ;

GINGERAS, TR .

NUCLEIC ACIDS RESEARCH, 1983, 11 (03) :837-851

[3] CLONING AND SEQUENCING THE HINFI RESTRICTION AND MODIFICATION GENES [J].

CHANDRASEGARAN, S ;

LUNNEN, KD ;

SMITH, HO ;

WILSON, GG .

GENE, 1988, 70 (02) :387-392

[4]

DAYHOFF MO, 1983, METHOD ENZYMOL, V91, P524

[5]

GREENE PJ, 1981, J BIOL CHEM, V256, P2143

[6] COMMON EVOLUTIONARY ORIGIN OF THE PHAGE-T4 DAM AND HOST ESCHERICHIA-COLI DAM DNA-ADENINE METHYLTRANSFERASE GENES [J].

HATTMAN, S ;

WILKINSON, J ;

SWINTON, D ;

SCHLAGMAN, S ;

MACDONALD, PM ;

MOSIG, G .

JOURNAL OF BACTERIOLOGY, 1985, 164 (02) :932-937

[7] PURIFICATION, CLONING AND SEQUENCE-ANALYSIS OF RSRI DNA METHYLTRANSFERASE - LACK OF HOMOLOGY BETWEEN 2 ENZYMES, RSRI AND ECORI, THAT METHYLATE THE SAME NUCLEOTIDE IN IDENTICAL RECOGNITION SEQUENCES [J].

KASZUBSKA, W ;

AIKEN, C ;

OCONNOR, CD ;

GUMPORT, RI .

NUCLEIC ACIDS RESEARCH, 1989, 17 (24) :10403-10425

[8]

KITA K, 1989, J BIOL CHEM, V264, P5751

[9] SEQUENCE MOTIFS CHARACTERISTIC OF DNA[CYTOSINE-N4]METHYLTRANSFERASES - SIMILARITY TO ADENINE AND CYTOSINE-C5 DNA-METHYLASES [J].

KLIMASAUSKAS, S ;

TIMINSKAS, A ;

MENKEVICIUS, S ;

BUTKIENE, D ;

BUTKUS, V ;

JANULAITIS, A .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :9823-9832

[10] GENETIC-BASIS OF THE COMPLEMENTARY DPNI AND DPNII RESTRICTION SYSTEMS OF STREPTOCOCCUS-PNEUMONIAE - AN INTERCELLULAR CASSETTE MECHANISM [J].

LACKS, SA ;

MANNARELLI, BM ;

SPRINGHORN, SS ;

GREENBERG, B .

CELL, 1986, 46 (07) :993-1000

← 1 2 3 4 5 →