C-13 NMR ANALYSIS OF METHIONINE SULFOXIDE IN PROTEIN

被引：9

作者：

COHEN, JS ^{[1
]}

YARIV, J ^{[1
]}

KALB, AJ ^{[1
]}

JACOBSON, L ^{[1
]}

SHECHTER, Y ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT BIOPHYS ISOTOPE RES & ORGAN CHEM, REHOVOT 76100, ISRAEL

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 03期

关键词：

D O I：

10.1016/0165-022X(79)90033-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The 13Cε{lunate} NMR signal of methionine sulfoxide is 22.6 ppm downfield from that of methionine. This affords a method by which the extent of methionine oxidation can be determined in intact protein. We demonstrate the utility of this approach with ß-galactosidase enriched with 13C in its methionine methyls. © 1979.

引用

页码：145 / 151

页数：7

共 11 条

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