CLONING AND CHARACTERIZATION OF THE RAT GLUTATHIONE-PEROXIDASE GENE

被引：37

作者：

HO, YS ^{[1
]}

HOWARD, AJ ^{[1
]}

机构：

[1] DUKE UNIV, MED CTR,DEPT MED,DIV ALLERGY CRIT CARE & RESP MED, MOLEC BIOL LAB, DURHAM, NC 27710 USA

来源：

FEBS LETTERS | 1992年 / 301卷 / 01期

关键词：

GENOMIC CLONING; S1 NUCLEASE MAPPING; PRIMER EXTENSION; REGULATORY SEQUENCE;

D O I：

10.1016/0014-5793(92)80198-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The increased activity of glutathione peroxidase (GSHPx) in rat lungs is associated with the development of tolerance of the animals to hyperoxia. o understand further the regulation of expression of this enzyme, the molecular structure of the corresponding rat gene was characterized. The rat GSHPx gene consists of two exons interrupted by a single intron of 217 base pairs. The same initiation sites for transcription were found to be utilized in both lung and liver. The promoter of the GSHPx gene contains neither a 'TATA' box nor a 'CAAT' box. Instead, it comprises two copies of Sp1 binding motif and one copy of AP-2 binding motif. These features of the promoter may offer a clue to the mechanisms by which the expression of this gene is controlled.

引用

页码：5 / 9

页数：5

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