APPLICATIONS OF ELECTROSPRAY MASS-SPECTROMETRY TO STUDIES ON THE STRUCTURAL-PROPERTIES OF RIBONUCLEASE-A AND RIBONUCLEASE-B

被引：12

作者：

CAMILLERI, P ^{[1
]}

HASKINS, NJ ^{[1
]}

RUDD, PM ^{[1
]}

SAUNDERS, MR ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,INST GLYCOBIOL,OXFORD OX1 3QU,ENGLAND

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 05期

关键词：

D O I：

10.1002/rcm.1290070505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Deuterium-exchange experiments were performed on ribonuclease A (RNase A) and its glycosylated form, ribonuclease B (RNase B), using electrospray ionization mass spectrometry. The number of exchangeable protons was found to be similar for both forms of the enzyme, indicating that the oligosaccharide moiety on RNase B does not exert any influence on protein conformation. Four main glycoform populations were identified in RNase B, each of which was homogeneous and associated with one phosphate group. Spectra for RNase A were heterogeneous due to the presence of 0-3 phosphate groups bound to the enzyme.

引用

页码：332 / 335

页数：4

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