SCID MOUSE PRE-B CELLS WITH INTRACELLULAR MU-CHAINS - ANALYSIS OF RECOMBINASE ACTIVITY AND IGH GENE REARRANGEMENTS

被引：13

作者：

KOTLOFF, DB ^{[1
]}

BOSMA, MJ ^{[1
]}

RUETSCH, NR ^{[1
]}

机构：

[1] FOX CHASE CANC CTR,INST CANC RES,7701 BURHOLME AVE,PHILADELPHIA,PA 19111

来源：

INTERNATIONAL IMMUNOLOGY | 1993年 / 5卷 / 04期

关键词：

V(D)J RECOMBINATION; PRE-B CELL CLONES; LEAKY SCID MICE;

D O I：

10.1093/intimm/5.4.383

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Four Pre-B cell clones with intracellular mu chains were recovered from individual leaky scid mice by transformation of bone marrow or peritoneal cells with Abelson murine leukemia virus. Three clones were derived from independent bone marrow cell cultures. These express the defective scid recombinase activity and contain truncated mu chains resulting from abnormal and/or incomplete (D to J only) gene rearrangements. A fourth clone was obtained from a peritoneal cell culture and may represent a revertant. It expresses a recombinase activity indistinguishable from that of wild-type cells; one of its rearranged IgH alleles (VDJ+) encodes a normal size mu chain, the other is non-productively rearranged (VDJ-).

引用

页码：383 / 391

页数：9

共 36 条

[1] MULTIPLE IMMUNOGLOBULIN HEAVY-CHAIN GENE TRANSCRIPTS IN ABELSON MURINE LEUKEMIA VIRUS-TRANSFORMED LYMPHOID-CELL LINES [J].

ALT, FW ;

ROSENBERG, N ;

ENEA, V ;

SIDEN, E ;

BALTIMORE, D .

MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (04) :386-400

[2] ISOLATION OF SCID PRE-B CELLS THAT REARRANGE KAPPA-LIGHT CHAIN GENES - FORMATION OF NORMAL SIGNAL AND ABNORMAL CODING JOINS [J].

BLACKWELL, TK ;

MALYNN, BA ;

POLLOCK, RR ;

FERRIER, P ;

COVEY, LR ;

FULOP, GM ;

PHILLIPS, RA ;

YANCOPOULOS, GD ;

ALT, FW .

EMBO JOURNAL, 1989, 8 (03) :735-742

[3] GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].

BLIN, N ;

STAFFORD, DW .

NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2303-2308

[4] EVIDENCE OF FUNCTIONAL LYMPHOCYTES IN SOME (LEAKY) SCID MICE [J].

BOSMA, GC ;

FRIED, M ;

CUSTER, RP ;

CARROLL, A ;

GIBSON, DM ;

BOSMA, MJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (03) :1016-1033

[5] THE SCID MOUSE MUTANT - DEFINITION, CHARACTERIZATION, AND POTENTIAL USES [J].

BOSMA, MJ ;

CARROLL, AM .

ANNUAL REVIEW OF IMMUNOLOGY, 1991, 9 :323-350

[6]

CARROLL AM, 1989, J IMMUNOL, V143, P1087

[7] DETECTION AND CHARACTERIZATION OF FUNCTIONAL T-CELLS IN MICE WITH SEVERE COMBINED IMMUNE-DEFICIENCY [J].

CARROLL, AM ;

BOSMA, MJ .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (12) :1965-1971

[8] LYMPHOCYTE-T DEVELOPMENT IN SCID MICE IS ARRESTED SHORTLY AFTER THE INITIATION OF T-CELL RECEPTOR DELTA-GENE RECOMBINATION [J].

CARROLL, AM ;

BOSMA, MJ .

GENES & DEVELOPMENT, 1991, 5 (08) :1357-1366

[9]

CUSTER RP, 1985, AM J PATHOL, V120, P464

[10]

FEENEY AJ, 1992, J IMMUNOL, V149, P222

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