CELL CYCLE-DEPENDENT DISTRIBUTION OF TELOMERES, CENTROMERES, AND CHROMOSOME-SPECIFIC SUBSATELLITE DOMAINS IN THE INTERPHASE NUCLEUS OF MOUSE LYMPHOCYTES

Fluorescence in situ hybridization and 3-D image analysis were combined to study the distribution of specific chromosome subdomains through the course of the cell cycle in cultured mouse lymphocytes. DNA probes specific for major satellite DNA, minor satellite DNA, telomeric DNA and to chromosome X-, 8-, and 14-specific subsatellite DNA sequences were used. We demonstrate that a redistribution of the chromatin occurs during the cell cycle in the interphase nucleus, and that the profile of the rearrangement is highly dependent on the nature of the domain considered (centromeres, telomeres, or subsatellite regions). However, the relative arrangement of chromosome homologs to each other does not appear to be spatially defined or regulated. © 1993 Academic Press, Inc.