(CAC)(5) DETECTS DNA FINGERPRINTS AND SEQUENCES HOMOLOGOUS TO GENE TRANSCRIPTS IN RICE

被引：28

作者：

GUPTA, VS ^{[1
]}

RAMAKRISHNA, W ^{[1
]}

RAWAT, SR ^{[1
]}

RANJEKAR, PK ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV BIOCHEM SCI,PLANT MOLEC BIOL UNIT,POONA 411008,INDIA

来源：

BIOCHEMICAL GENETICS | 1994年 / 32卷 / 1-2期

关键词：

RICE; SIMPLE REPETITIVE SEQUENCE; DNA FINGERPRINTING; (CAC)(5); POLY(A)(+); TRANSCRIPTS;

D O I：

10.1007/BF00557234

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present report the potential of (CAC)(5) is demonstrated for DNA fingerprinting in rice. Based on the fingerprint data, (CAC)(5) was estimated to differentiate up to 6.3 x 10(8) genotypes of rice. In a search for homologous sequences in rice transcripts, four oligonucleotide probes, namely, (CAC)(5), (GATA)(4), (GACA)(4), and (TG)(10), were used. Among these probes, (CAC)(5) showed hybridization to total rice RNA in a tissue-specific manner; while a band at 1.2 kb was common in both seed and leaf RNA, there were additional prominent bands at 1.9 and 2.3 kb in seed and leaf RNA, respectively. When (CAC)(5) was hybridized to poly(A)(+) RNA, in addition to a band at 1.2 kb in seed and leaf hybridization was observed only in seed to heterogeneously sized RNAs.

引用

页码：1 / 8

页数：8

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