CLONING AND EXPRESSION OF GENES-CODING FOR CARBOHYDRATE DEGRADING ENZYMES OF ANAEROCELLUM THERMOPHILUM IN ESCHERICHIA-COLI

被引：8

作者：

BOLSHAKOVA, EV

PONOMARIEV, AA

NOVIKOV, AA

SVETLICHNYI, VA

VELIKODVORSKAYA, GA

机构：

[1] Russian Acad Sci, Inst Molec Genet, Moscow, 123182

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 202卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. Clones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The genes of one beta-1,4-glucanase and one xylanase are probably located in the same region of A.thermophilum chromosome. (C) 1994 Academic Press, Inc.

引用

页码：1076 / 1080

页数：5

共 9 条

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