SIGNIFICANCE OF AN UNUSUALLY LOW K(M) FOR GLUTATHIONE IN GLUTATHIONE TRANSFERASES OF THE ALPHA-CLASS, MU-CLASS AND PI-CLASS

1. Interactions of glutathione transferases (GST) of the alpha, mu and pi classes with glutathione (GSH) and glutathione conjugates (GS-X) are in contrast with those of a GST of the theta class (GST5-5). 2. GST 5-5 has a K(m) for GSH of approx. 5 mM. Thus K(m)/ambient [GSH] is approx. 1, within the range of K(m)/ambient [s] of glycolytic enzymes. GSTs of the alpha, mu and pi classes yield much lower values of K(m) for GSH (approx. 0.1 mm) hence K(m)/ambient [s] is significantly lower than those of most (non-GST) enzymes (p<0.025). 3. GSTs of the alpha, mu and pi classes are sensitive to inhibition by GS-X (i.e. product) and GS-X analogues. GST 5-5 is not. 4. Rate enhancements up to 10(10), similar to an average enzyme (10(8)-10(12)), are seen in catalysis by GST 5-5, but not in catalysis by GSTs of alpha, mu and pi classes (>10(7)). 5. Comparisons of primary structure indicate that theta class GSTs may have a decreased binding of the glu-alpha-amino- and gly-COO--groups of GSH compared with GSTs of the other classes. 6. It is concluded that GSTs of alpha, mu and pi classes have evolved towards increased product binding at the expense of catalytic efficiency. Thus GSH is uniquely utilized both as a nucleophile and a 'tag' which can be used to bind and sequester product particularly during GSH-depletion. This interpretation unifies the catalytic and binding properties of these GSTs and alters their perceived role in detoxication.