FATTY ACID INTERACTION WITH HYDROXYLATING ENZYME SYSTEM OF RAT LIVER MICROSOMES

被引：38

作者：

ORRENIUS, S

THOR, H

机构：

[1] Biokemiska Institutionen, Kungliga Universitetet Odengatan, Stockholm

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 9卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00624.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Addition of a low concentration of laurate (Km= 6 μM) to rat liver microsomes in the presence of NADPH was found to stimulate the rate of NADPH disappearance parallel to the formation of oxidized metabolites, mainly ω‐hydroxy laurate. When added to a suspension of liver microsomes laurate gave rise to the formation of a “type I” spectral change indicating an interaction of the fatty acid with the microsomal mixed function oxidase. Whereas laurate present in an optimal concentration for ω‐oxidation caused a competitive inhibition of the oxidative demethylation of drugs, this activity as well as the ω‐oxidation activity was almost completely extinguished in the presence of a high fatty acid concentration (approx. 3 mM). At this concentration laurate had a solubilizing effect on the microsomes which was associated with a conversion of cytochrome P‐450 to the enzymically inactive P‐420 form. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：415 / &

共 26 条

[1]

CONNEY AH, 1968, J BIOL CHEM, V243, P3912

[2] PHOTOCHEMICAL ACTION SPECTRUM OF TERMINAL OXIDASE OF MIXED FUNCTION OXIDASE SYSTEMS [J].