DETECTION OF ALBUMIN-SECRETING HEPATOCYTES AT THE SINGLE CELL LEVEL BY A PROTEIN-A PLAQUE-ASSAY

被引:16
作者
SMITH, CIE
HAMMARSTROM, L
机构
[1] Department of Immunobiology, Karolinska Institute, Stockholm
关键词
D O I
10.1002/eji.1830090713
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In a complement‐dependent assay, using protein A‐coupled erythrocytes as target cells and anti‐albumin immunoglobulin (Ig) as a developing serum, it was possible to detect single albumin‐secreting hepatocytes as plaque‐forming cells. Plasmacytoma cells were detected using anti‐Ig antibodies and did not form plaques with anti‐albu‐ min Ig. This method can potentially be used for detecting any cell‐secreting products in sufficient amounts. Copyright © 1979 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
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页码:570 / 571
页数:2
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