INSECTICIDE PRODUCTION BY RECOMBINANT BACILLUS-SUBTILIS-1A96 IN FED-BATCH CULTURE WITH CONTROL OF GLUCOSE-CONCENTRATION

被引：15

作者：

CAYUELA, C ^{[1
]}

KAI, K ^{[1
]}

PARK, YS ^{[1
]}

IIJIMA, S ^{[1
]}

KOBAYASHI, T ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC ENGN,DEPT BIOTECHNOL,FURO CHO,CHIKUSA KU,NAGOYA 46401,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 75卷 / 05期

关键词：

D O I：

10.1016/0922-338X(93)90139-Y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fed-batch culture of Bacillus subtilis strain 1A96 containing an insecticide gene integrated into the chromosome was performed by controlling glucose concentration at about 2 g/l and 9 g/l. The cell concentration and insecticidal potency obtained at 2 g/l of glucose were 104 g-dry cell/l and 394 kIU/ml, which were 2.2 and 1.6 times, respectively, as high as those of the fed-batch culture with control of glucose concentration at 9 g/l.

引用

页码：383 / 386

页数：4

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