ASSESSMENT OF UREASE INHIBITION ASSAYS FOR MEASURING TOXICITY OF ENVIRONMENTAL-SAMPLES

被引：47

作者：

JUNG, K ^{[1
]}

BITTON, G ^{[1
]}

KOOPMAN, B ^{[1
]}

机构：

[1] UNIV FLORIDA,DEPT ENVIRONM ENGN SCI,GAINESVILLE,FL 32611

来源：

WATER RESEARCH | 1995年 / 29卷 / 08期

基金：

美国国家科学基金会;

关键词：

UREASE; HEAVY METALS; TOXICITY; INDUSTRIAL WASTE-WATER;

D O I：

10.1016/0043-1354(94)00353-9

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We evaluated the potential of free and immobilized urease for assaying toxicity of environmental samples. Free urease was sensitive to heavy metals and insensitive to organic toxicants. Toxicity of industrial wastewaters and process water, as determined via free urease assay, was correlated with the heavy metal content of these samples. High background (blank) absorbance was encountered in samples containing ammonia. The background exceeded the linear range of the assay at ammonia nitrogen concentrations exceeding 8 mg/l. Immobilizing urease on glass beads was considered as a means of overcoming the problem of ammonia interference. Immobilized enzyme was unfortunately not sensitive to heavy metals. The potential of free urease for toxicity testing cannot be fully realized until a means of overcoming ammonia interference is developed.

引用

页码：1929 / 1933

页数：5

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