AMINOGLYCOSIDE-3''-ADENYLTRANSFERASE CONFERS RESISTANCE TO SPECTINOMYCIN AND STREPTOMYCIN IN NICOTIANA-TABACUM

被引：46

作者：

SVAB, Z ^{[1
]}

HARPER, EC ^{[1
]}

JONES, JDG ^{[1
]}

MALIGA, P ^{[1
]}

机构：

[1] DNA PLANT TECHNOL CORP,OAKLAND,CA 94608

来源：

PLANT MOLECULAR BIOLOGY | 1990年 / 14卷 / 02期

关键词：

Aminoglycoside-3″-adenyltransferase; chimeric gene; Nicotiana tabacum; spectinomycin resistance; streptomycin resistance; transgenic plant;

D O I：

10.1007/BF00018560

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin. © 1990 Kluwer Academic Publishers.

引用

页码：197 / 205

页数：9

共 33 条

[1] A CHIMAERIC ANTIBIOTIC-RESISTANCE GENE AS A SELECTABLE MARKER FOR PLANT-CELL TRANSFORMATION [J].