STRUCTURE OF THE GLYCOSYL-PHOSPHATIDYLINOSITOL MEMBRANE ANCHOR OF ACETYLCHOLINESTERASE FROM THE ELECTRIC ORGAN OF THE ELECTRIC-FISH, TORPEDO-CALIFORNICA

被引：22

作者：

MEHLERT, A

VARON, L

SILMAN, I

HOMANS, SW

FERGUSON, MAJ

机构：

[1] UNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLAND

[2] WEIZMANN INST SCI,DEPT NEUROBIOL,IL-76100 REHOVOT,ISRAEL

来源：

BIOCHEMICAL JOURNAL | 1993年 / 296卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj2960473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the glycan moiety of the glycosyl-phosphatidylinositol (GPI) membrane anchor from Torpedo californica (electric fish) electric-organ acetylcholinesterase was solved using n.m.r., methylation analysis and chemical and enzymic micro-sequencing. Two structures were found to be present: Glcalpha1-2Manalpha1-2Manalpha1-6Manalpha1-4GlcNalpha1-6myo-inositol and Glcalpha1-2Manalpha1-2Manalpha1-6(GaINAcbeta1-4)Manalpha1-4GlcNalpha1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.

引用

页码：473 / 479

页数：7

共 38 条

[1] POTOCYTOSIS - SEQUESTRATION AND TRANSPORT OF SMALL MOLECULES BY CAVEOLAE [J].