INTEGRATION SITE OF HUMAN PAPILLOMAVIRUS TYPE-18 DNA IN CHROMOSOME BAND-8Q22.1 OF C4-1 CERVICAL-CARCINOMA - DNASE-I HYPERSENSITIVITY AND METHYLATION OF CELLULAR FLANKING SEQUENCES

被引：16

作者：

GALLEGO, MI

ZIMONJIC, DB

POPESCU, NC

DIPAOLO, JA

LAZO, PA

机构：

[1] INST SALUD CARLOS III,CTR NACL BIOL CELULAR & RETROVIRUS,UNIDAD GENET MOLEC,E-28220 MAJADAHONDA,SPAIN

[2] NCI,DIV CANC ETIOL,BIOL LAB,BETHESDA,MD 20892

来源：

GENES CHROMOSOMES & CANCER | 1994年 / 9卷 / 01期

关键词：

D O I：

10.1002/gcc.2870090106

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The C4-1 cell line derived from a non-keratinizing squamous cell carcinoma of the uterine cervix contains integrated human papillomavirus-18 DNA. Fluorescence in situ hybridization of C4-1 cells demonstrated a single viral integration site at 8q22.1 on a derivative chromosome originating from an 8q;12q translocation. 8q22 is a site of chromosome fragility and is also recombinogenic in several human malignancies. DNase I hypersensitivity of the integration site was studied with a cellular flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4-1 and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells. (C) 1994 Wiley-Uss, Inc.

引用

页码：28 / 32

页数：5

共 32 条

[1]

BARLETTA C, 1991, CANCER RES, V51, P3821

[2]

BARTHOLOMEW C, 1989, ONCOGENE, V4, P529

[3] REGIONAL CHROMOSOME LOCALIZATION OF HUMAN PAPILLOMAVIRUS INTEGRATION SITES NEAR FRAGILE SITES, ONCOGENES, AND CANCER CHROMOSOME BREAKPOINTS [J].