SEQUENCING, CHROMOSOMAL INACTIVATION, AND FUNCTIONAL EXPRESSION IN ESCHERICHIA-COLI OF PPSR, A GENE WHICH REPRESSES CAROTENOID AND BACTERIOCHLOROPHYLL SYNTHESIS IN RHODOBACTER-SPHAEROIDES

被引:94
作者
PENFOLD, RJ [1 ]
PEMBERTON, JM [1 ]
机构
[1] UNIV QUEENSLAND,DEPT MICROBIOL,ST LUCIA,QLD 4072,AUSTRALIA
关键词
D O I
10.1128/JB.176.10.2869-2876.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sequencing of a DNA fragment that causes trans suppression of bacteriochlorophyll and carotenoid levels in Rhodobacter sphaeroides revealed two genes: orf-192 and ppsR. The ppsR gene alone is sufficient for photopigment suppression. Inactivation of the R. sphaeroides chromosomal copy of ppsR results in overproduction of both bacteriochlorophyll and carotenoid pigments. The deduced 464-amino-acid protein product of ppsR is homologous to the CrtJ protein of Rhodobacter capsulatus and contains a helix-turn-helix domain that is found in various DNA-binding proteins. Removal of the helix-turn-helix domain renders PpsR nonfunctional. The promoter of ppsR is located within the coding region of the upstream orf-192 gene. When this promoter is replaced by a lacZ promoter, ppsR is expressed in Escherichia coli. An R. sphaeroides DNA fragment carrying crtD', -E, and -F and bchC, -X, -Y, and -Z' exhibited putative promoter activity in E. coli. This putative promoter activity could be suppressed by PpsR in both E. coli and R. sphaeroides. These results suggest that PpsR is a transcriptional repressor. It could potentially act by binding to a putative regulatory palindrome found in the 5' flanking regions of a number of R. sphaeroides and R. capsulatus photosynthesis genes.
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页码:2869 / 2876
页数:8
相关论文
共 56 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]  
ARMSTRONG GA, 1990, J BIOL CHEM, V265, P8329
[3]   NUCLEOTIDE-SEQUENCE, ORGANIZATION, AND NATURE OF THE PROTEIN PRODUCTS OF THE CAROTENOID BIOSYNTHESIS GENE-CLUSTER OF RHODOBACTER-CAPSULATUS [J].
ARMSTRONG, GA ;
ALBERTI, M ;
LEACH, F ;
HEARST, JE .
MOLECULAR & GENERAL GENETICS, 1989, 216 (2-3) :254-268
[4]   CONTROL OF PHOTOSYSTEM GENES IN RHODOBACTER-CAPSULATUS [J].
BAUER, C ;
BUGGY, J ;
MOSLEY, C .
TRENDS IN GENETICS, 1993, 9 (02) :56-60
[5]   THE SUPEROPERONAL ORGANIZATION OF GENES FOR PIGMENT BIOSYNTHESIS AND REACTION CENTER PROTEINS IS A CONSERVED FEATURE IN RHODOBACTER-CAPSULATUS - ANALYSIS OF OVERLAPPING BCHB AND PUHA TRANSCRIPTS [J].
BAUER, CE ;
BUGGY, JJ ;
YANG, ZM ;
MARRS, BL .
MOLECULAR & GENERAL GENETICS, 1991, 228 (03) :433-444
[6]   GENETIC ANALYSES OF PHOTOPIGMENT BIOSYNTHESIS IN EUBACTERIA - A GUIDING LIGHT FOR ALGAE AND PLANTS [J].
BAUER, CE ;
BOLLIVAR, DW ;
SUZUKI, JY .
JOURNAL OF BACTERIOLOGY, 1993, 175 (13) :3919-3925
[7]  
BOWEN ARS, 1985, PLASMIDS BACTERIA
[8]   PLASMID VECTORS FOR THE SELECTION OF PROMOTERS [J].
BROSIUS, J .
GENE, 1984, 27 (02) :151-160
[9]   BCHFNBH BACTERIOCHLOROPHYLL SYNTHESIS GENES OF RHODOBACTER-CAPSULATUS AND IDENTIFICATION OF THE 3RD SUBUNIT OF LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IN BACTERIA AND PLANTS [J].
BURKE, DH ;
ALBERTI, M ;
HEARST, JE .
JOURNAL OF BACTERIOLOGY, 1993, 175 (08) :2414-2422
[10]   ONE-STEP PREPARATION OF COMPETENT ESCHERICHIA-COLI - TRANSFORMATION AND STORAGE OF BACTERIAL-CELLS IN THE SAME SOLUTION [J].
CHUNG, CT ;
NIEMELA, SL ;
MILLER, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2172-2175