GENOMIC ORGANIZATION OF THE HUMAN CYSTATHIONINE BETA-SYNTHASE GENE - EVIDENCE FOR VARIOUS CDNAS

被引：22

作者：

CHASSE, JF ^{[1
]}

PALY, E ^{[1
]}

PARIS, D ^{[1
]}

PAUL, V ^{[1
]}

SINET, PM ^{[1
]}

KAMOUN, P ^{[1
]}

LONDON, J ^{[1
]}

机构：

[1] HOP NECKER ENFANTS MALAD,CNRS,URA 1335,F-75743 PARIS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 211卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1886

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A CBS cDNA isolated from an adult liver cDNA library was cloned and sequenced. The 5' untranslated region (5'-UTR) contains a sequence which is only partially common (88nt) with that previously published (3). It is expressed as a 2.5 kb species mostly in liver and pancreas and faintly in brain, heart, kidney and lung and as a 3.7 kb in pancreas and liver. The human cystathionine beta-synthase gene (CBS) was isolated from a cosmid genomic library and its structure was determined. The CBS gene is at least 23 kb long and is composed of 17 exons. The organization of the human gene is different from that of the rat especially in the 5'-UTR. In the rat gene the ATG is present in exon 1, conversely in the human gene first the ATG is present in exon 3 and second the 5'-UTR contains two different exons 1 (E1a and E1b) linked with exon 2. (C) 1995 Academic Press, Inc.

引用

页码：826 / 832

页数：7

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