KINETIC-STUDIES OF FORMALDEHYDE BINDING IN TISSUE

被引:100
作者
HELANDER, KG
机构
[1] Laboratory of Membrane Biology, Center for Ulcer Research and Education, University of California, Los Angeles, CA
关键词
FORMALDEHYDE; FIXATION; KINETICS;
D O I
10.3109/10520299409106282
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Specimens of rabbit liver were fixed for various periods up to 6 days in buffered C-14-formaldehyde. Binding of the isotope reached a plateau after fixation for approximately 24 hr; the half-maximal binding level was reached after approximately 100 min. Formaldehyde binding at 37 C was faster than at 25 C, and faster at pH 7.0 than at pH 4.0. During rinsing of the fixed tissue in water for up to 26 days there was a progressive decrease in isotope content to 10-20% of the prerinse level, indicating that formaldehyde fixation is a reversible process.
引用
收藏
页码:177 / 179
页数:3
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