A METHOD FOR DEMONSTRATION OF HYDROLYTIC ENZYMES WITH SEQUENTIAL CYTOPHOTOMETRIC MEASUREMENT OF DNA NUCLEAR HISTONE AND TOTAL PROTEIN

In cytophotometric analyses of nuclear proteins of mixed cell populations such as lymph nodes, bone marrow or spleen it is frequently difficult to place a single cell to be explored into a precise category of cell types. This factor of uncertainty concerning the classification of cells may substantially reduce the value of those measurements. In order to eliminate or diminish such sources of error a method is described which by means of cytochemical enzyme tracing allows for a reliable classification of single cells to be examined prior to the real quantitative analysis of the same cell without impairment of the results of the measurements. © 1969 Springer-Verlag.