2-PHASE CULTURE FOR THE ENHANCED PRODUCTION OF BENZOPHENANTHRIDINE ALKALOIDS IN CELL-SUSPENSIONS OF ESCHSCHOLTZIA-CALIFORNICA

被引：24

作者：

BYUN, SY

PEDERSEN, H

CHIN, CK

机构：

[1] RUTGERS STATE UNIV, DEPT CHEM & BIOCHEM ENGN, NEW BRUNSWICK, NJ 08903 USA

[2] RUTGERS STATE UNIV, DEPT HORT & FORESTRY, NEW BRUNSWICK, NJ 08903 USA

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 10期

关键词：

benzophenanthridine alkaloid; Eschscholtzia californica; Papaveraceae; California poppy; suspension culture; two-phase culture; yeast elicitor;

D O I：

10.1016/0031-9422(90)80172-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Accumulation of the benzophenanthridine alkaloids sanguinarine, chelerythrine, chelirubine and macarpine occurred in suspension cultures ofEschscholtzia californica. An enhanced production of some of these alkaloids was found in a two-phase culture system and in a system with elicitation in conjunction with two-phase culture. A versatile and practical accumulation phase for culture ofE. californica was discovered. This phase was composed of silicone fluid, which accumulated a large amount of the alkaloid and greatly increased alkaloid production compared to cultures grown in its absence. Furthermore, elicitation in combination with two-phase culture additionally increased alkaloid production 10-fold. © 1990 Pergamon Press plc.

引用

页码：3135 / 3139

页数：5

共 12 条

[1] 2-PHASE CULTURE - A METHOD FOR THE ISOLATION OF LIPOPHILIC SUBSTANCES FROM PLANT SUSPENSION-CULTURES [J].