SIMPLE AND RAPID PURIFICATION OF BREVIN

被引：103

作者：

KUROKAWA, H

FUJII, W

OHMI, K

SAKURAI, T

NONOMURA, Y

机构：

[1] Department of Pharmacology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92342-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brevin or plasma gelsolin, a calcium dependent actin-binding and actin-severing protein, was purified from bovine plasma by a very rapid and simple procedure; ammonium sulfate fractionation and only one step of anion exhange column chromatography by a convenient use. It takes only 24 hrs to complete all the procedure. The purity of brevin prepared by this method was more than 95 % on SDS-PAGE and total recovery was much better than previous preparation methods. This brevin preparation has about 8 isomers on 2-D PAGE and strong severing activity on F-actin under electron microscopic observation. © 1990.

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收藏

页码：451 / 457

页数：7

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