IDENTIFICATION AND NUCLEOTIDE-SEQUENCE OF THE ACINETOBACTER-CALCOACETICUS ENCODED TRPE GENE

被引：6

作者：

HASPEL, G ^{[1
]}

HUNGER, M ^{[1
]}

SCHMUCKER, R ^{[1
]}

HILLEN, W ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG,INST MIKROBIOL & BIOCHEM,LEHRSTUHL MIKROBIOL,STAUDSTR 5,W-8520 ERLANGEN,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 220卷 / 03期

关键词：

Acinetobacter calcoaceticus; Anthranilate synthase; trpE gene; Tryptophan biosynthesis;

D O I：

10.1007/BF00391756

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The trpE gene from Acinetobacter calcoaceticus encoding the anthranilate synthase component I was cloned, identified by deletion analysis and sequenced. It encodes a predicted polypeptide of 497 amino acids with a calculated molecular weight of 55323. Its primary structure shows 49% identical amino acids with the enzyme from Clostridium thermocellum, 45% with that of Thermus thermophilus and only 35% with that of Escherichia coli. The codon usage of the trpE genes encoding the most homologous enzymes differs greatly indicating selection for amino acid maintainance. The homologies are clustered in the C-terminal 200 amino acids of the sequences indicating that this part is important for enzymic activity. © 1990 Springer-Verlag.

引用

页码：475 / 477

页数：3

共 17 条

[1] RHIZOBIUM-MELILOTI ANTHRANILATE SYNTHASE GENE - CLONING, SEQUENCE, AND EXPRESSION IN ESCHERICHIA-COLI [J].