MOLECULAR CHARACTERIZATION OF THE GENES ENCODING ACETOHYDROXY ACID SYNTHASE IN THE CYANOBACTERIUM SPIRULINA-PLATENSIS

被引：11

作者：

MILANO, A ^{[1
]}

DEROSSI, E ^{[1
]}

ZANARIA, E ^{[1
]}

BARBIERATO, L ^{[1
]}

CIFERRI, O ^{[1
]}

RICCARDI, G ^{[1
]}

机构：

[1] DIPARTIMENTO GENET & MICROBIOL A BUZZATI TRAVERSO, VIA ABBIATEGRASSO 207, I-27100 PAVIA, ITALY

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷

关键词：

D O I：

10.1099/00221287-138-7-1399

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The enzyme acetohydroxy acid synthase (AHS), which catalyses the first common step in the biosynthesis of isoleucine, leucine and valine, has been demonstrated to be present in Spirulina platensis in two isoenzymic forms. The complete nucleotide sequences of the genes ilv X and ilv W encoding these two enzymes have been determined. Sequence analysis revealed the presence of two open reading frames, of 1836 and 1737 nucleotides for ilv X and ilv W, respectively. The predicted amino acid sequences of the two isoenzymes, compared with the Synechococcus PCC 7942 AHS enzyme and the large subunits of the Escherichia coli AHSI, II, III isoenzymes, revealed a notable degree of similarity. A small subunit has not been identified for either of the S. platensis AHS isoenzymes. Analysis by Northern blot hybridization demonstrated that the ilv X and ilv W genes are transcribed to give mRNA species of approximately 2.15 kb and 1.95 kb, respectively.

引用

页码：1399 / 1408

页数：10

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