ISOLATION OF 353 NOTL-LINKING CLONES AND 62 DNA MARKERS (DXS607-DXS668) FROM HUMAN-CHROMOSOME XQ24-]QTER

Through restriction enzyme digestion of 1,784 cosmid DNAs derived from human chromosome region Xq24 --> qter, we identified 353 NotI-linking cosmids (11 individual groups of overlapping NotI-linking cosmids at Xq24 --> q25,6 groups at Xq26 --> q27, and 19 groups at Xq28). Sixty-two of the 3 5 3 clones (DXS607-DXS668) contained multiple rare-cutter sites, including multiple SacII sites within a 2-kb region, as well as one to six sites for BssHII, MluI, NotI, or NruI.