REPAIR OF UV-INDUCED (6-4) PHOTOPRODUCTS IN NUCLEOSOME CORE DNA

被引:46
作者
SUQUET, C [1 ]
MITCHELL, DL [1 ]
SMERDON, MJ [1 ]
机构
[1] UNIV TEXAS,MD ANDERSON CANC CTR,SMITHVILLE,TX 78957
关键词
D O I
10.1074/jbc.270.28.16507
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using radioimmunoassays, we examined rates of removal of UV-induced pyrimidine-pyrimidone (6-4) photoproducts ((6-4)PDs) and cyclobutane pyrimidine dimers (CPDs) from 146-base pair nucleosome core DNA (and 166-base pair chromatosome DNA) of confluent human diploid fibroblasts. Dose-response experiments indicate that the yield of (6-4)PDs in core DNA is about 30% that of CPDs in the UV dose range of 0-200 J/m(2). Repair experiments indicate that, at 40 J/m(2), (6-4)PDs are removed much faster (similar to 75% in 2 h) from nucleosome core (and chromatosome) DNA than CPDs (10-15% in 2 h). A slow rate of removal of CPDs is also observed when the UV dose is reduced to 10 J/m(2) (i.e. even when the level of CPDs is less than that of (6-4)PDs at 40 J/m(2)). These results indicate that (a) the accessibility of repair proteins to (6-4)PDs in nucleosomes is markedly different than their accessibility to CPDs and/or (b) repair enzymes are much more efficient at incising and removing (6-4)PDs than CPDs in human chromatin.
引用
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页码:16507 / 16509
页数:3
相关论文
共 34 条
  • [1] PHOTOREACTIVATION IMPLICATES CYCLOBUTANE DIMERS AS THE MAJOR PROMUTAGENIC UVB LESIONS IN YEAST
    ARMSTRONG, JD
    KUNZ, BA
    [J]. MUTATION RESEARCH, 1992, 268 (01): : 83 - 94
  • [2] Bohr VA, 1988, DNA REPAIR LABORATOR, V3, P347
  • [3] BRASH DE, 1988, PHOTOCHEM PHOTOBIOL, V48, P59
  • [4] PHOTOCHEMISTRY OF NUCLEIC-ACIDS IN CELLS
    CADET, J
    ANSELMINO, C
    DOUKI, T
    VOITURIEZ, L
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 15 (04) : 277 - 298
  • [5] TRANSCRIPTION AND NUCLEOTIDE EXCISION-REPAIR - REFLECTIONS, CONSIDERATIONS AND RECENT BIOCHEMICAL INSIGHTS
    FRIEDBERG, EC
    BARDWELL, AJ
    BARDWELL, L
    WANG, ZG
    DIANOV, G
    [J]. MUTATION RESEARCH, 1994, 307 (01): : 5 - 14
  • [6] UV-INDUCED FORMATION OF PYRIMIDINE DIMERS IN NUCLEOSOME CORE DNA IS STRONGLY MODULATED WITH A PERIOD OF 10.3 BASES
    GALE, JM
    NISSEN, KA
    SMERDON, MJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) : 6644 - 6648
  • [7] UV INDUCED (6-4) PHOTOPRODUCTS ARE DISTRIBUTED DIFFERENTLY THAN CYCLOBUTANE DIMERS IN NUCLEOSOMES
    GALE, JM
    SMERDON, MJ
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1990, 51 (04) : 411 - 417
  • [8] PHOTOFOOTPRINT OF NUCLEOSOME CORE DNA IN INTACT CHROMATIN HAVING DIFFERENT STRUCTURAL STATES
    GALE, JM
    SMERDON, MJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (04) : 949 - 958
  • [9] STRANDED IN AN ACTIVE GENE
    HANAWALT, P
    MELLON, I
    [J]. CURRENT BIOLOGY, 1993, 3 (01) : 67 - 69
  • [10] HARM W., 1980, BIOL EFFECTS ULTRAVI