SIMPLIFIED TEST FOR DETECTING THE RESISTANCE OF HERPES-SIMPLEX VIRUS TO ACYCLOVIR

被引：11

作者：

AGUT, H

AUBIN, JT

INGRAND, D

BLANC, S

CLAYTON, AL

CHANTLER, SM

HURAUX, JM

机构：

[1] WELLCOME FDN LTD,PARIS,FRANCE

[2] WELLCOME RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF MEDICAL VIROLOGY | 1990年 / 31卷 / 03期

关键词：

enzyme amplified ELISA; HSV; screening resistance test;

D O I：

10.1002/jmv.1890310307

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The detection of herpes simplex virus (HSV) antigen by means of an enzyme amplified ELISA was investigated for rapid screening of acyclovir (ACV) resistance. Vero cell monolayers were inoculated with various strains of HSV and incubated in the presence of different concentrations of ACV. When cytopathic effect was present, the culture supernatants were tested by ELISA. The absorbance values were found to correlate with the results of virus yield and plaque reduction assays. The comparison between absorbance values obtained in the presence of 10 μM ACV and in the absence of drug provided the basis for a simplified sensitivity test. The use of a single ACV concentration allowed discrimination between ACV‐resistant and ACV‐sensitive reference strains, the detection of ACV‐resistant virus mixed in the proportion of 10% with ACV‐sensitive virus, and a study of the emergence of an ACV‐resistant virus population in serial samples taken from experimental rabbit keratitis. The simplified susceptibility assay is a sensitive and convenient method for rapid screening of HSV resistance to ACV. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：209 / 214

页数：6

共 19 条

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