SIMPLIFIED TEST FOR DETECTING THE RESISTANCE OF HERPES-SIMPLEX VIRUS TO ACYCLOVIR

被引:11
作者
AGUT, H
AUBIN, JT
INGRAND, D
BLANC, S
CLAYTON, AL
CHANTLER, SM
HURAUX, JM
机构
[1] WELLCOME FDN LTD,PARIS,FRANCE
[2] WELLCOME RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND
关键词
enzyme amplified ELISA; HSV; screening resistance test;
D O I
10.1002/jmv.1890310307
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The detection of herpes simplex virus (HSV) antigen by means of an enzyme amplified ELISA was investigated for rapid screening of acyclovir (ACV) resistance. Vero cell monolayers were inoculated with various strains of HSV and incubated in the presence of different concentrations of ACV. When cytopathic effect was present, the culture supernatants were tested by ELISA. The absorbance values were found to correlate with the results of virus yield and plaque reduction assays. The comparison between absorbance values obtained in the presence of 10 μM ACV and in the absence of drug provided the basis for a simplified sensitivity test. The use of a single ACV concentration allowed discrimination between ACV‐resistant and ACV‐sensitive reference strains, the detection of ACV‐resistant virus mixed in the proportion of 10% with ACV‐sensitive virus, and a study of the emergence of an ACV‐resistant virus population in serial samples taken from experimental rabbit keratitis. The simplified susceptibility assay is a sensitive and convenient method for rapid screening of HSV resistance to ACV. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company
引用
收藏
页码:209 / 214
页数:6
相关论文
共 19 条
  • [1] AGUT H, 1988, PRESSE MED, V17, P753
  • [2] EVALUATION OF HERPES-SIMPLEX VIRUS SUSCEPTIBILITY TO ACYCLOVIR USING AN ENZYME-LINKED IMMUNOSORBENT-ASSAY
    ANDRE, PM
    NARBONNE, CH
    DONNIO, PY
    RUFFAULT, A
    FAUCONNIER, B
    [J]. ANNALES DE L INSTITUT PASTEUR-VIROLOGY, 1988, 139 (02): : 185 - 195
  • [3] COMPARISON OF 2 METHODS IN THE DETERMINATION OF THE SENSITIVITY OF 84 HERPES-SIMPLEX VIRUS (HSV) TYPE-1 AND TYPE-2 CLINICAL ISOLATES TO ACYCLOVIR AND ALPHA-INTERFERON
    BRISEBOIS, JJ
    DUMAS, VM
    JONCAS, JH
    [J]. ANTIVIRAL RESEARCH, 1989, 11 (02) : 67 - 76
  • [4] HERPES-SIMPLEX VIRUS DETECTION BY ELISA - EFFECT OF ENZYME AMPLIFICATION, NATURE OF LESION SAMPLED AND SPECIMEN TREATMENT
    CLAYTON, AL
    ROBERTS, C
    GODLEY, M
    BEST, JM
    CHANTLER, SM
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1986, 20 (01) : 89 - 97
  • [5] COLLINS P, 1988, AM J MED, V85, P129
  • [6] HSV1 STRAIN SENSITIVITY IN EXPERIMENTAL RABBIT KERATITIS - EVOLUTION UNDER REPEATED TOPICAL IDU ADMINISTRATIONS
    DENIS, J
    LANGLOIS, M
    ELKAIM, M
    AMIEL, C
    AYMARD, M
    HURAUX, JM
    [J]. CURRENT EYE RESEARCH, 1987, 6 (01) : 39 - 45
  • [7] ACYCLOVIR-RESISTANT HERPES-SIMPLEX VIRUS-INFECTIONS IN PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME
    ERLICH, KS
    MILLS, J
    CHATIS, P
    MERTZ, GJ
    BUSCH, DF
    FOLLANSBEE, SE
    GRANT, RM
    CRUMPACKER, CS
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1989, 320 (05) : 293 - 296
  • [8] FIELD HJ, 1986, HUMAN HERPESVIRUS IN, P177
  • [9] COMPARATIVE METHODS FOR DETECTION OF THYMIDINE KINASE-DEFICIENT HERPES-SIMPLEX VIRUS TYPE-1 STRAINS
    HARMENBERG, J
    SUNDQVIST, VA
    GADLER, H
    LEVEN, B
    BRANNSTROM, G
    WAHREN, B
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1986, 30 (04) : 570 - 573
  • [10] RAPID METHOD FOR DETERMINING THE SUSCEPTIBILITY OF HERPES-SIMPLEX VIRUS TO ACYCLOVIR
    HOWELL, CL
    MILLER, MJ
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1984, 2 (02) : 77 - 84