ISOLATION OF A COMPLEMENTARY-DNA ENCODING THE BEAN PR4 CHITINASE - AN ACIDIC ENZYME WITH AN AMINO-TERMINUS CYSTEINE-RICH DOMAIN

被引:61
作者
MARGISPINHEIRO, M
METZBOUTIGUE, MH
AWADE, A
DETAPIA, M
LERET, M
BURKARD, G
机构
[1] INST BIOL MOLEC PLANTES,12 RUE GEN ZIMMER,F-67084 STRASBOURG,FRANCE
[2] INST BIOL MOLEC & CELLULAIRE,F-67084 STRASBOURG,FRANCE
关键词
BEAN CHITINASE CDNA; CYSTEINE-RICH DOMAIN; PLANT DEFENSE; CHEMICAL STRESS;
D O I
10.1007/BF00039499
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The amino acid sequences of peptides generated by trypsin and chymotrypsin digestions of the acidic PR4 chitinase from bean were determined. Oligonucleotide primers derived from this sequence were used to synthesize a PR4 chitinase-specific probe by PCR-amplification. This probe allowed the isolation of cDNA clones encoding PR4 chitinase that have been sequenced. This acidic and extracellular chitinase shows some homology to the basic isoform from the same plant, and differs from other known acidic chitinases by the presence of an amino-terminal cysteine-rich domain. Southern blot analysis of bean genomic DNA revealed that PR4 chitinase is encoded by a single gene.
引用
收藏
页码:243 / 253
页数:11
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