INDUCED ELECTROSTATIC POTENTIALS ON ANTIGEN-ANTIBODY COMPLEXES FOR BIOANALYTICAL APPLICATIONS

被引:18
作者
LEE, CS
HUANG, PY
AYRES, DM
机构
[1] Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore, Maryland 21228, Baltimore County Campus
关键词
D O I
10.1021/ac00005a016
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A concept for directly sensing the antigen-antibody binding event is presented. This direct sensing mechanism was studied with the use of protein A-IgG complex as the model system. IgG carries a net charge except at its isoelectric point (pI). The binding of IgG to protein A would contribute to an additional electric field around protein A. The presence of this additional electric field will therefore change the local pH near the protein A-IgG complex. This change in the local pH can then be readily observed by measuring the fluorescence property of fluorescein molecules conjugated with protein A before and after the introduction of IgG. The preliminary theoretical analyses and experimental results of protein A, fluorescein conjugate, and IgG complex are discussed.
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页码:464 / 467
页数:4
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