RECOMBINANT PFS25 PROTEIN OF PLASMODIUM-FALCIPARUM ELICITS MALARIA TRANSMISSION-BLOCKING IMMUNITY IN EXPERIMENTAL-ANIMALS

被引：177

作者：

BARR, PJ ^{[1
]}

GREEN, KM ^{[1
]}

GIBSON, HL ^{[1
]}

BATHURST, IC ^{[1
]}

QUAKYI, IA ^{[1
]}

KASLOW, DC ^{[1
]}

机构：

[1] NIAID,PARASIT DIS LAB,BETHESDA,MD 20892

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1991年 / 174卷 / 05期

关键词：

D O I：

10.1084/jem.174.5.1203

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pfs25 is a sexual stage antigen of Plasmodium falciparum that is expressed on the surface of zygote and ookinete forms of the parasite. Monoclonal antibodies directed against native Pfs25 can block completely the development of P. falciparum oocysts in the midgut of the mosquito vector. Thus, this 25-kD protein is a potential vaccine candidate for eliciting transmission-blocking immunity in inhabitants of malaria endemic regions. We have synthesized, by secretion from yeast, a polypeptide analogue of Pfs25 that reacts with conformation-dependent monoclonal antibodies, and elicits transmission-blocking antibodies when used to immunize mice and monkeys in conjunction with a muramyl tripeptide adjuvant. Our results suggest the further evaluation of recombinant DNA-derived Pfs25 in transmission-blocking vaccination studies in humans.

引用

页码：1203 / 1208

页数：6

共 17 条

[1] PRIMING OF CD4+ T-CELLS SPECIFIC FOR CONSERVED REGIONS OF HUMAN-IMMUNODEFICIENCY-VIRUS GLYCOPROTEIN GP120 IN HUMANS IMMUNIZED WITH A RECOMBINANT ENVELOPE PROTEIN
ABRIGNANI, S
MONTAGNA, D
JEANNET, M
WINTSCH, J
HAIGWOOD, NL
SHUSTER, JR
STEIMER, KS
CRUCHAUD, A
STAEHELIN, T
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) : 6136 - 6140
[2] IMMUNOGENICITY OF RECOMBINANT PLASMODIUM-FALCIPARUM SERA PROTEINS IN RODENTS
BARR, PJ
INSELBURG, J
GREEN, KM
KANSOPON, J
HAHM, BK
GIBSON, HL
LEENG, CT
BZIK, DJ
LI, W
BATHURST, IC
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 45 (01) : 159 - 170
[3] BARR PJ, 1988, J BIOL CHEM, V263, P16471
[4] BATHURST IC, 1992, IN PRESS BIOTECHNOL
[5] BRAKE AJ, 1984, P NATL ACAD SCI-BIOL, V81, P4642, DOI 10.1073/pnas.81.15.4642
[6] CHARACTERIZATION OF EPITOPES ON THE 25 KD PROTEIN OF THE MACROGAMETES ZYGOTES OF PLASMODIUM-FALCIPARUM
FRIES, HCW
LAMERS, MBAC
SMITS, MA
PONNUDURAI, T
MEUWISSEN, JHET
[J]. PARASITE IMMUNOLOGY, 1989, 11 (01) : 31 - 45
[7] CHARACTERIZATION OF RECOMBINANT HUMAN EPIDERMAL GROWTH-FACTOR PRODUCED IN YEAST
GEORGENASCIMENTO, C
GYENES, A
HALLORAN, SM
MERRYWEATHER, J
VALENZUELA, P
STEIMER, KS
MASIARZ, FR
RANDOLPH, A
[J]. BIOCHEMISTRY, 1988, 27 (02) : 797 - 802
[8] GILL GN, 1984, J BIOL CHEM, V259, P7755
[9] SAFETY AND IMMUNOGENICITY OF A PLASMODIUM-VIVAX SPOROZOITE VACCINE
GORDON, DM
COSGRIFF, TM
SCHNEIDER, I
WASSERMAN, GF
MAJARIAN, WR
HOLLINGDALE, MR
CHULAY, JD
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 42 (06) : 527 - 531
[10] AMINO TERMINAL ACETYLATION OF AUTHENTIC HUMAN CU,ZN SUPEROXIDE-DISMUTASE PRODUCED IN YEAST
HALLEWELL, RA
MILLS, R
TEKAMPOLSON, P
BLACHER, R
ROSENBERG, S
OTTING, F
MASIARZ, FR
SCANDELLA, CJ
[J]. BIO-TECHNOLOGY, 1987, 5 (04): : 363 - 366

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