REPLICATION ORIGINS AND PAUSE SITES IN SEA-URCHIN MITOCHONDRIAL-DNA

被引:27
作者
MAYHOOK, AG [1 ]
RINALDI, AM [1 ]
JACOBS, HT [1 ]
机构
[1] UNIV PALERMO, DIPARTIMENTO BIOL CELLULARE & SVILUPPO, I-90123 PALERMO, ITALY
关键词
D O I
10.1098/rspb.1992.0046
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have used a combination of one- and two-dimensional agarose gel electrophoresis, and solution hybridization to strand-specific probes, to map the replication origin of sea urchin mitochondrial and to investigate the structure of replication intermediates. These assays are consistent with replication initiating unidirectionally from the D-loop region by D-loop expansion, as in vertebrates. A prominent site of initiation of lagging-strand synthesis lies at, or near to, the boundary between the genes for ATPase 6 and COIII, which is also close to a pause site for leading-strand synthesis. These findings suggest a role for pause sites in the regulation of mitochondrial transcription and replication, possibly involving template-binding proteins.
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页码:85 / 94
页数:10
相关论文
共 38 条
[1]  
Bachmann B. J., 1987, ESCHERICHIA COLI SAL, V2, P807
[2]   MECHANISM OF MITOCHONDRIAL-DNA REPLICATION IN MOUSE L-CELLS - ASYNCHRONOUS REPLICATION OF STRANDS, SEGREGATION OF CIRCULAR DAUGHTER MOLECULES, ASPECTS OF TOPOLOGY AND TURNOVER OF AN INITIATION SEQUENCE [J].
BERK, AJ ;
CLAYTON, DA .
JOURNAL OF MOLECULAR BIOLOGY, 1974, 86 (04) :801-824
[3]   THE LOCALIZATION OF REPLICATION ORIGINS ON ARS PLASMIDS IN SACCHAROMYCES-CEREVISIAE [J].
BREWER, BJ ;
FANGMAN, WL .
CELL, 1987, 51 (03) :463-471
[4]   A REPLICATION FORK BARRIER AT THE 3' END OF YEAST RIBOSOMAL-RNA GENES [J].
BREWER, BJ ;
FANGMAN, WL .
CELL, 1988, 55 (04) :637-643
[5]   A NOVEL GENE ORDER IN THE PARACENTROTUS-LIVIDUS MITOCHONDRIAL GENOME [J].
CANTATORE, P ;
ROBERTI, M ;
MORISCO, P ;
RAINALDI, G ;
GADALETA, MN ;
SACCONE, C .
GENE, 1987, 53 (01) :41-54
[6]   REPLICATION PRIMING AND TRANSCRIPTION INITIATE FROM PRECISELY THE SAME SITE IN MOUSE MITOCHONDRIAL-DNA [J].
CHANG, DD ;
HAUSWIRTH, WW ;
CLAYTON, DA .
EMBO JOURNAL, 1985, 4 (06) :1559-1567
[7]   ROLES FOR A PROMOTER AND RNA PROCESSING IN THE SYNTHESIS OF MITOCHONDRIAL DISPLACEMENT-LOOP STRANDS [J].
CHANG, DD ;
FISHER, RP ;
CLAYTON, DA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 909 (02) :85-91
[8]  
CHASE JW, 1972, DEV BIOL, V27, P504, DOI 10.1016/0012-1606(72)90189-3
[9]   A TRIDECAMER DNA-SEQUENCE SUPPORTS HUMAN MITOCHONDRIAL RNA 3'-END FORMATION INVITRO [J].
CHRISTIANSON, TW ;
CLAYTON, DA .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (10) :4502-4509
[10]   INVITRO TRANSCRIPTION OF HUMAN MITOCHONDRIAL-DNA - ACCURATE TERMINATION REQUIRES A REGION OF DNA-SEQUENCE THAT CAN FUNCTION BIDIRECTIONALLY [J].
CHRISTIANSON, TW ;
CLAYTON, DA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) :6277-6281