P-32 LABELED AND BIOTIN-LABELED INVITRO TRANSCRIBED CRNA PROBES FOR THE DETECTION OF POTATO SPINDLE TUBER VIROID AND CHRYSANTHEMUM STUNT VIROID

被引：12

作者：

CANDRESSE, T

MACQUAIRE, G

BRAULT, V

MONSION, M

DUNEZ, J

机构：

[1] Station de Pathologie végétale, INRA, Centre de Recherches de Bordeaux, BP 131

来源：

RESEARCH IN VIROLOGY | 1990年 / 141卷 / 01期

关键词：

cRNA; Dot-blot; Probes; CSV; PSTV; RNA; Phytopathology; Viroid;

D O I：

10.1016/0923-2516(90)90059-R

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Replacing nick-translated DNA probes by in vitro transcribed complementary RNA (cRNA) probes considerably increased the sensitivity of dot-blot detection tests of potato spindle tuber viroid and chrysanthemum stunt viroid. As compared to the limit of detection of 5-10 pg of viroid obtained with 32P-labelled DNA probes, cRNA probes allow the detection of less than 1 pg of pure viroid. When labelled with biotin by incorporation of biotin-labelled ribonucleotides, the cRNA probes have a limit of detection of approximately 5 pg of purified viroid. © 1990.

引用

页码：97 / 107

页数：11

共 18 条

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