INDUCTION OF BETA-1,3-GLUCANASE IN BARLEY IN RESPONSE TO INFECTION BY FUNGAL PATHOGENS

被引：34

作者：

JUTIDAMRONGPHAN, W

ANDERSEN, JB

MACKINNON, G

MANNERS, JM

SIMPSON, RS

SCOTT, KJ

机构：

[1] Department of Biochemistry, University of Queensland

[2] Department of Plant Pathology, Agricultural University, DK-1871 Frederiksberg C

[3] Commonwealth Scientific and Industrial Research Organization, Division of Tropical Crops, Pastures-UQ Plant Pathology Unit

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1991年 / 4卷 / 03期

关键词：

DISEASE RESISTANCE;

D O I：

10.1094/MPMI-4-234

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence of a partial cDNA clone corresponding to an mRNA induced in leaves of barley (Hordeum vulgare) by infection with fungal pathogens matched almost perfectly with that of a cDNA clone coding for beta-1,3-glucanase isolated from the scutellum of barley. Western blot analysis of intercellular proteins from near-isogenic barley lines inoculated with the powdery mildew fungus (Erysiphe graminis f. sp. hordei) showed a strong induction of glucanase in all inoculated lines but was most pronounced in two resistant lines. These data were confirmed by beta-1,3-glucanase assays. The barley cDNA was used as a hybridization probe to detect mRNAs in barley, wheat (Triticum aestivum), rice (Oryza sativus), and sorghum (Sorghum bicolor), which are induced by infection with the necrotrophic pathogen Bipolaris sorokiniana. These results demonstrate that activation of beta-1,3-glucanase genes may be a general response of cereals to infection by fungal pathogens.

引用

页码：234 / 238

页数：5

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