CROSS-LINKING PROTEINS TO NUCLEIC-ACIDS BY ULTRAVIOLET-LASER IRRADIATION

被引：95

作者：

PASHEV, IG ^{[1
]}

DIMITROV, SI ^{[1
]}

ANGELOV, D ^{[1
]}

机构：

[1] BULGARIAN ACAD SCI,INST SOLID STATE PHYS,BU-1113 SOFIA,BULGARIA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1991年 / 16卷 / 09期

关键词：

D O I：

10.1016/0968-0004(91)90133-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ultraviolet (UV) irradiation can initiate complex formation between proteins and DNA or RNA and so can be used to study such interactions. However, crosslink formation by standard UV light sources can take up to several hours. More recently, a beam of monochromatic UV light from a laser has been used to initiate crosslinking in nano- and picosecond time intervals. As noted in an earlier TIBS article 'the advantages of short pulse times and high-energy fluxes should make this a valuable technique in the future' 1. In this review we characterize laser-induced crosslinking and explore the applications of this method.

引用

页码：323 / 326

页数：4

共 19 条

[11]

MARKOVITZ A, 1972, BIOCHIM BIOPHYS ACTA, V281, P522, DOI 10.1016/0005-2787(72)90153-0

[12] 2-QUANTUM UV PHOTOCHEMISTRY OF NUCLEIC-ACIDS - COMPARISON WITH CONVENTIONAL LOW-INTENSITY UV PHOTOCHEMISTRY AND RADIATION-CHEMISTRY [J].

NIKOGOSYAN, DN .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1990, 57 (02) :233-299

[13]

PARADISO PR, 1979, J BIOL CHEM, V254, P4739

[14]

Shetlar M.D., 1980, PHOTOCHEMICAL AND PHOTOBIOLOGICAL REVIEWS, V5, P105

[15] A MIXED PHOTOPRODUCT OF THYMINE AND CYSTEINE - 5-S-CYSTEINE, 6-HYDROTHYMINE [J].

SMITH, KC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 39 (06) :1011-&

[16] DOSE DEPENDENT DECREASE IN EXTRACTABILITY OF DNA FROM BACTERIA FOLLOWING IRRADIATION WITH ULTRAVIOLET LIGHT OR WITH VISIBLE LIGHT PLUS DYE [J].

SMITH, KC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1962, 8 (03) :157-&

[17] INTERACTIONS OF ACETYLATED HISTONES WITH DNA AS REVEALED BY UV LASER-INDUCED HISTONE-DNA CROSSLINKING [J].

STEFANOVSKY, VY ;

DIMITROV, SI ;

ANGELOV, D ;

PASHEV, IG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (01) :304-310

[18] LASER-INDUCED CROSSLINKING OF HISTONES TO DNA IN CHROMATIN AND CORE PARTICLES - IMPLICATIONS IN STUDYING HISTONE-DNA INTERACTIONS [J].

STEFANOVSKY, VY ;

DIMITROV, SI ;

RUSSANOVA, VR ;

ANGELOV, D ;

PASHEV, IG .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :10069-10081

[19] PROTEIN - DNA CROSS-LINKING [J].

WELSH, J ;

CANTOR, CR .

TRENDS IN BIOCHEMICAL SCIENCES, 1984, 9 (12) :505-508

← 1 2 →