COMPARISON OF INDIRECT CALORIMETRY AND A NEW BREATH C-13/C-12 RATIO METHOD DURING STRENUOUS EXERCISE

被引:99
作者
ROMIJN, JA
COYLE, EF
HIBBERT, J
WOLFE, RR
机构
[1] UNIV TEXAS,MED BRANCH,SHRINERS BURNS INST,METAB UNIT,610 TEXAS AVE,GALVESTON,TX 77550
[2] UNIV TEXAS,MED BRANCH,DEPT ANESTHESIOL,GALVESTON,TX 77550
[3] UNIV TEXAS,MED BRANCH,DEPT SURG,GALVESTON,TX 77550
[4] UNIV TEXAS,DEPT KINESIOL & HLTH,HUMAN PERFORMANCE LAB,AUSTIN,TX 78712
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 01期
关键词
STABLE ISOTOPES; MASS SPECTROMETRY; SUBSTRATE OXIDATION;
D O I
10.1152/ajpendo.1992.263.1.E64
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
A new stable isotope method for the determination of substrate oxidation rates in vivo is described and compared with indirect calorimetry at rest and during high-intensity exercise (30 min at 80-85% maximal O2 uptake capacity) in six well-trained cyclists. This method uses the absolute ratios of C-13/C-12 in expired air, endogenous glucose, fat, and protein in addition to O2 consumption and is independent Of CO2 production (V(CO2)). Carbohydrate and fat oxidation rates at rest, calculated by both methods, were not significantly different. During exercise the breath C-13/C-12 ratio increased and reached a steady state after 15-20 min. Carbohydrate oxidation rates during exercise were 39.4 +/- 5.2 and 41.7 +/- 5.7 mg.kg-1-min-1 [not significant (NS)], and fat oxidation rates were 7.3 +/- 1.3 and 6.9 +/- 1.2 mg.kg-1.min-1 (NS), using indirect calorimetry and the breath ratio method, respectively. We conclude that the breath C-13/C-12 ratio method can be used to calculate substrate oxidation under different conditions, such as the basal state and exercise. In addition, the results obtained by this new method support the validity of the underlying assumption that indirect calorimetry regards V(CO2) as a reflection of tissue CO2 production, during exercise in trained subjects, even up to 80-85% maximal O2 uptake.
引用
收藏
页码:E64 / E71
页数:8
相关论文
共 21 条
[1]   INFLUENCE OF INCREASED METABOLIC-RATE ON [C-13]BICARBONATE WASHOUT KINETICS [J].
BARSTOW, TJ ;
COOPER, DM ;
SOBEL, EM ;
LANDAW, EM ;
EPSTEIN, S .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (01) :R163-R171
[2]   CHANGES IN BREATH (CO2/CO2)-C-13-C-12 CONSEQUENT TO EXERCISE AND HYPOXIA [J].
BARSTOW, TJ ;
COOPER, DM ;
EPSTEIN, S ;
WASSERMAN, K .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (02) :936-942
[3]   EFFECTS OF FETAL INSULIN INFUSION ON GLUCOSE KINETICS IN PREGNANT SHEEP - A COMPARTMENTAL ANALYSIS [J].
BLOCH, CA ;
BANACH, W ;
LANDT, K ;
DEVASKAR, S ;
SPERLING, MA .
AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 251 (04) :E448-E455
[4]   EFFECT OF EXERCISE AND RECOVERY ON MUSCLE PROTEIN-SYNTHESIS IN HUMAN-SUBJECTS [J].
CARRARO, F ;
STUART, CA ;
HARTL, WH ;
ROSENBLATT, J ;
WOLFE, RR .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (04) :E470-E476
[5]   MUSCLE GLYCOGEN UTILIZATION DURING PROLONGED STRENUOUS EXERCISE WHEN FED CARBOHYDRATE [J].
COYLE, EF ;
COGGAN, AR ;
HEMMERT, MK ;
IVY, JL .
JOURNAL OF APPLIED PHYSIOLOGY, 1986, 61 (01) :165-172
[6]   THE THEORETICAL BASES OF INDIRECT CALORIMETRY - A REVIEW [J].
FERRANNINI, E .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1988, 37 (03) :287-301
[7]   CALCULATION OF SUBSTRATE OXIDATION RATES INVIVO FROM GASEOUS EXCHANGE [J].
FRAYN, KN .
JOURNAL OF APPLIED PHYSIOLOGY, 1983, 55 (02) :628-634
[8]  
HULTMAN E, 1967, ACTA MED SCAND, V182, P109
[9]   PREVALENCE OF C-13 IN RESPIRATORY CARBON DIOXIDE AS AN INDICATOR OF TYPE OF ENDOGENOUS SUBSTRATE . CHANGE FROM LIPID TO CARBOHYDRATE DURING RESPIRATORY RISE IN POTATO SLICES [J].
JACOBSON, BS ;
SMITH, BN ;
EPSTEIN, S ;
LATIES, GG .
JOURNAL OF GENERAL PHYSIOLOGY, 1970, 55 (01) :1-&
[10]   POST-EXERCISE KETOSIS [J].
KOESLAG, JH ;
NOAKES, TD ;
SLOAN, AW .
JOURNAL OF PHYSIOLOGY-LONDON, 1980, 301 (APR) :79-90