SPECIFIC INDUCTION OF A FUNCTIONAL ENDOGENOUS D2 SHORT DOPAMINE RECEPTOR IN GH4C1 CELLS

被引：12

作者：

ALLARD, S ^{[1
]}

LAPOINTE, S ^{[1
]}

FALARDEAU, P ^{[1
]}

机构：

[1] CHUL,DEPT ONTOGENESE & GENET MOLEC,RC9300,2705 BOUL LAURIER,ST FOY G1V 4G2,PQ,CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1696

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In contrast to lactotrophs, tumoral pituitary cells like GH3 and GH4C1 lack expression of dopamine D2short and D2long receptors. In GH4C1 cells, we observed that the expression of only the short isoform of D2 receptor can be induced after transfection with a plasmid which confers resistance to neomycin (pRSVNeo). High levels of fully functional D2short receptor were obtained in GH4C1 following transfection (528fmol/mg protein). Sequence, pharmacology and coupling of the induced-D2 receptor do not show any difference with the cloned rat D2 short receptor. © 1993 Academic Press, Inc.

引用

页码：801 / 807

页数：7

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