CANCER CELL BINDING TO E-SELECTIN TRANSFECTED HUMAN ENDOTHELIA

被引：11

作者：

MERWIN, JR ^{[1
]}

MADRI, JA ^{[1
]}

LYNCH, M ^{[1
]}

机构：

[1] YALE UNIV, DEPT PATHOL, NEW HAVEN, CT 06510 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91560-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human endothelial cells were transiently transfected with E-Selectin which enabled us to study tumor cell/endothelial interactions following engagement of E-Selectin without the added complications of metabolic stimulation, morphological changes, and/or up regulation of other adhesion molecules due to cytokine induction. Similar results were received from in vitro binding studies and FACS analyses on both Tumor Necrosis Factor-alpha activated and E-Selectin transfected endothelial cells. These data suggest that this methodology is appropriate for dissecting the individual activities of E-selectin while minimizing the participation of other adhesion molecules, thereby allowing us to develop a better understanding of the role of E-Selectin and endothelia in metastatic disease. © 1992.

引用

页码：315 / 323

页数：9

共 22 条

[1] GRANULE MEMBRANE PROTEIN-140 (GMP140) BINDS TO CARCINOMAS AND CARCINOMA-DERIVED CELL-LINES [J].