FLAVONOID INDUCERS OF NODULATION GENES STIMULATE RHIZOBIUM-FREDII USDA257 TO EXPORT PROTEINS INTO THE ENVIRONMENT

被引:48
作者
KRISHNAN, HB [1 ]
PUEPPKE, SG [1 ]
机构
[1] UNIV MISSOURI,DEPT PLANT PATHOL,COLUMBIA,MO 65211
关键词
COMMON NOD GENES; SYMBIOSIS PLASMID;
D O I
10.1094/MPMI-6-107
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genistein, an isoflavone from the roots of soybean (Glycine max), is among the most powerful inducers of the expression of nodulation genes in Rhizobium fredii USDA257, a nitrogen-fixing symbiont of this plant. We show here that concentrations of this compound as low as 100-500 nM also stimulate cells of USDA257 to excrete five major proteins, of molecular masses of 54, 39, 36, 20, and 8.9 kDa. Other known inducers of nodulation genes in R. fredii, including luteolin and naringenin, also elicit accumulation of this set of proteins in USDA257, but the noninducers biochanin A and quercetin are inactive in induction of protein secretion. [S-35]Methionine is incorporated into each of the proteins, but none contains detectable polysaccharide. Export of an array of proteins in response to nodulation gene inducers is characteristic of R. fredii and depends on the presence of an intact symbiosis plasmid. R. leguminosarum by.phaseoli, Bradyrhizobium japonicum, and broad host range Rhizobium sp. strain NGR234 each elaborate a single genistein-induced extracellular protein, but the response is lacking when several other nitrogen-fixing species are exposed to their nodulation gene inducers. The N-terminal sequence of the 36-kDa protein, MetTyrSerSerLysThrGlySerAlaDerGlnSerThr, differs from that of all known nodulation genes, and the conservation of the N-terminal methionine indicates that this protein probably is exported without N-terminal processing.
引用
收藏
页码:107 / 113
页数:7
相关论文
共 63 条
[1]  
AKIYAMA T, 1987, J BIOL CHEM, V262, P5592
[2]   RHIZOBIUM-JAPONICUM USDA 191 HAS 2 NODD GENES THAT DIFFER IN PRIMARY STRUCTURE AND FUNCTION [J].
APPELBAUM, ER ;
THOMPSON, DV ;
IDLER, K ;
CHARTRAIN, N .
JOURNAL OF BACTERIOLOGY, 1988, 170 (01) :12-20
[3]   6 NODULATION GENES OF NOD BOX LOCUS-4 IN RHIZOBIUM-MELILOTI ARE INVOLVED IN NODULATION SIGNAL PRODUCTION - NODM CODES FOR D-GLUCOSAMINE SYNTHETASE [J].
BAEV, N ;
ENDRE, G ;
PETROVICS, G ;
BANFALVI, Z ;
KONDOROSI, A .
MOLECULAR & GENERAL GENETICS, 1991, 228 (1-2) :113-124
[4]   CALCIUM REGULATES GROWTH OF RHIZOBIUM-FREDII AND ITS ABILITY TO NODULATE SOYBEAN CV PEKING [J].
BALATTI, PA ;
KRISHNAN, HB ;
PUEPPKE, SG .
CANADIAN JOURNAL OF MICROBIOLOGY, 1991, 37 (07) :542-548
[5]   IDENTIFICATION OF NORTH-AMERICAN SOYBEAN LINES THAT FORM NITROGEN-FIXING NODULES WITH RHIZOBIUM-FREDII USDA257 [J].
BALATTI, PA ;
PUEPPKE, SG .
CANADIAN JOURNAL OF PLANT SCIENCE, 1992, 72 (01) :49-55
[6]  
BALATTI PA, 1992, PLANT PHYSIOL BIOCH, V30, P193
[7]  
Bender G. L., 1988, Molecular Plant-Microbe Interactions, V1, P259, DOI 10.1094/MPMI-1-259
[8]   CHARACTERIZATION AND SEQUENCE OF FOLLICLE CELL GENES SELECTIVELY EXPRESSED DURING VITELLINE MEMBRANE FORMATION IN DROSOPHILA [J].
BURKE, T ;
WARING, GL ;
POPODI, E ;
MINOO, P .
DEVELOPMENTAL BIOLOGY, 1987, 124 (02) :441-450
[9]   EFFECT OF INOCULATION AND NITROGEN ON ISOFLAVONOID CONCENTRATION IN WILD-TYPE AND NODULATION-MUTANT SOYBEAN ROOTS [J].
CHO, MJ ;
HARPER, JE .
PLANT PHYSIOLOGY, 1991, 95 (02) :435-442
[10]   LOCALIZATION AND SYMBIOTIC FUNCTION OF A REGION ON THE RHIZOBIUM-LEGUMINOSARUM SYM PLASMID PRL1JI RESPONSIBLE FOR A SECRETED, FLAVONOID-INDUCIBLE 50-KILODALTON PROTEIN [J].
DEMAAGD, RA ;
SPAINK, HP ;
PEES, E ;
MULDERS, IHM ;
WIJFJES, A ;
WIJFFELMAN, CA ;
OKKER, RJH ;
LUGTENBERG, BJJ .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :1151-1157