ISRADIPINE AFFECTS HISTAMINE-INDUCED CYTOSOLIC CA2+ MOVEMENTS IN HUMAN ENDOTHELIAL-CELLS

被引：14

作者：

IOUZALEN, L ^{[1
]}

DEVYNCK, MA ^{[1
]}

DAVIDDUFILHO, M ^{[1
]}

机构：

[1] UNIV PARIS 05,NECKER MED SCH,DEPT PHARMACOL,CNRS,URA 1482,PARIS,FRANCE

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1995年 / 289卷 / 02期

关键词：

ENDOTHELIAL CELLS; HUMAN; CA2+; CYTOSOLIC; ISRADIPINE; FURA-2; HISTAMINE;

D O I：

10.1016/0922-4106(95)90094-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Although endothelial actions of dihydropyridines remain controversial, isradipine has been observed to exert anti-atherosclerotic actions in which endothelium could be involved. This study was designed to investigate the direct effects of isradipine on cytosolic Ca2+ concentration in cultured human umbilical vein endothelial cells. Isradipine (from 10 nM to 1 mu M) had no effect on unstimulated cells but dose-dependently decreased both the transient [Ca2+](i) peak and the sustained increase induced by histamine. Its maximal effects were reached at 0.1 mu M. In the absence of Ca2+ influx or in depolarized cells, 1 mu M isradipine still significantly decreased the transient [Ca2+](i) peak (by 23 +/- 8% and 42 +/- 11%). Ca2+ influx induced by re-establishment of transmembrane Ca2+ gradient was also inhibited by isradipine, as was that induced by 1 mu M thapsigargin. These results demonstrate that isradipine is able to reduce both Ca2+ release from internal stores and the consequent Ca2+ entry in stimulated human endothelial cells.

引用

页码：189 / 195

页数：7

共 34 条

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